Franciscan Church in Drohiczyn, dedicated to the Assumption of the Blessed Virgin Mary – according to the monastery tradition recorded in 1797 by Father Karol Gaudenty Żero (1743–1802) in his work entitled "Fenix de pulveribus resuscitatus svaviter suam candit modulenam," the Franciscan convent in Drohiczyn was founded by Mikołaj Nassuta, mentioned in 1430 as the starost of Drohiczyn. The monks are said to have settled on a square known as "Narożnik" (Corner).
The first endowments for the monks were confirmed in 1407 by Duke Witold Kiejstutowicz, who also provided offerings from the castle. During the reign of Janusz I the Elder, Duke of Mazovia, a wooden monastery and church dedicated to the Assumption of the Blessed Virgin Mary were built in 1409. In 1451, the guardian was Father Jan of Ruda, and in 1470, Father Szymon of Kraków. In the years 1460–1470, funds for the monastery were donated by, among others, the Drohicz court assessor Maciej Miłkowski (d. ca. 1478) and his wife Katarzyna.
The Franciscan monastery was burned down by dissenters in 1583, and around 1595, the wooden church burned down. In 1595–1596, it was rebuilt thanks to the efforts of Father Mateusz Studziński, guardian, but in 1601, both buildings were destroyed by fire again. Another wooden Franciscan church was built in 1615 through the efforts of the guardian, Father Bartłomiej Klucziata vel Klimczata, and consecrated on October 4, 1618, by the titular bishop of Argivensis and suffragan of Lutsk, Father Stanisław Udrzycki (d. 1621). During this period, a new monastery building was constructed. In 1657, during the Swedish Deluge, following Misericordiae Sunday, the monastery was destroyed again, and several monks were murdered by Cossack, Transylvanian, and Swedish troops commanded by Magnus Gabriel de La Gardie.